Additional file 6.

File showing differences in frequency of CD44/CD24 cells in A1.8 cell line that were growing in monolayer as compared to spheroids. (A) Fluorescence-activated cell sorting (FACS) analysis of stem cell markers from unsorted A1.8 parental cells is compared with SC+ (CD44+/CD24-) and SC- (CD44-/CD24+) cells sorted by FACS after growing as monolayers in the third passage (P.3). (B) RP.1 parental and CD133+ and CD133- cells sorted and passaged as monolayer twice (P.2) before analysis. One of three independent experiments is shown.

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Wright et al. Breast Cancer Research 2008 10:R10   doi:10.1186/bcr1855